[The Effect of NT-1505 on a Membrane Structure of Endoplasmic Reticulum in vivo].

The effect of neuroprotector NT-1505 on endoplasmic reticulum membranes was studied. It was shown that the dynamics of changes in lipid and near-protein areas microviscosity of endoplasmic reticulum membranes at drug administration is of an antibate nature. This points to the absence of pathological disturbances in the membrane structure. Membrane microviscosity was measured by electron paramagnetic resonance spin labeling of 2,2,6,6-tetramethyl-4-capryloyl-oxylpiperidine-1-oxyl (lipid probe) and 5,6-benzo-2,2,6,6-tetramethyl-1,2,3,4-tetrahydro-γ-carboline-3-oxyl (near protein probe). To obtain more complete information about changes in membrane structure under the action of neuroprotector NT-1505 the temperature dependence of rotational diffusion correlation time was measured in the temperature range of 283-317 K (10-44 degrees C). The two structural transitions were characterized for both areas of .membranes of control group in temperature intervals 16-20 degrees C and 32-38 degrees C which were still present after NT-1505 introduction. Therefore, NT-1505 has no significant effect on membrane structure of the endoplasmic reticulum.

[Studying the effect of Dimebon and NT-1505 on microviscosity of mice brain synaptosomal membranes in vivo by EPR spin labeling method].

In this work membrane fluidity alterations in synaptosomes, isolated from mice brain tissue, at chronic administration of neuroprotectors Dimebon and NT-1505 in vivo were studied. Membrane microviscosity was measured by electron paramagnetic resonance spin labeling of 2,2,6,6-tetramet-hyl-4-capryloyl-oxylpiperidine-l-oxyl (lipid probe) and 5,6-benzo-2,2,6,6-tetramethyl-1,2,3,4-tetrahydro-gamma-carboline-3-oxyl (near protein probe). It was shown that the neuroprotectors Dimebon and NT-1505 affect a membrane structure. Despite the difference in membrane structures, fluidity of the lipid bilayer in time returned to control values.

[Changes in levels of p53 protein, immunoglobulin L-chains, and iron complexes in mice of leukosis strain AKR following low dose irradiation]. / Izmenenie soderzhaniia belka p53, L-tsepei immunoglobulinov i kompleksov zheleza pri obluchenii malymi dozami myshei leikoznoi linii AKR.

Changes in the content of protein p53 (regulator of the cell cycle) L-chains of immunoglobulins, and iron complexes (Fe2+) during the development of spontaneous leukosis in AKR mice and upon irradiation of animals with a dose of 1.2 cGy were studied by ESR spectroscopy, electrophoresis, and immunoblotting. It was found that irradiation leads to an increase in the incidence of leukoses in males by 7% and a decrease in life duration of females. A decrease in the content of protein p53 and L-chains in immunoglobulins in males and females was observed; however, in females, the decreases was less pronounced because the content of these proteins in females is naturally decreased. In mice irradiated with low doses at the age of three- to four months, a decrease in the amount of iron complexes at a later age (seven- to eight months) was registered. These data suggest that there is a relationship between the induction of protein p53 and the content of immunoglobulin L-chains in the blood serum of animals.

[Changes in the EPR spectra of the nitrosyl complexes of blood proteins in the low-intensity whole-body irradiation of mice]. / Izmenenie spektrov EPR nitrozil'nykh kompleksov gemoproteinov krovi pri nizkointensivnom total'nom obluchenii myshei.

After NO adding to mice blood and isolated erythrocytes ESR signal of nitrozyl complex HbNO (g = 2.07, g = 1.98) and NO-induced MetNg (g = 6.0) were registered. It was shown that the intensity of ESR spectra of these complexes increased after radiation of mice with a dose of 0.06, 0.6 and 5.4 cGy. Low-dose irradiation (0.6 and 0.06 cGy) caused the change in the form of ESR spectra of HbNO (g = 2.07), which is indicative of the shift from T-structure to R-structure and of the preferred formation of R-conformations of oxyhemoglobin in blood. It was found that dependence of NO-induced MetHb signal on irradiation dose is bimodal that may be connected with nonlinear response of the cells to irradiation and retarded adaptive response after radiation with low doses.

[EPR-spectroscopy of human blood preparations. Remote sequelae of the Chernobyl accident]. / EPR-spektroskopiia preparatov krovi cheloveka. Otdalennye posledstviia Chernobyl'skoi avarii.

It was shown, that as a result of Chernobyl accident the intensity of the EPR-signal of hemoproteins and ferroporphyrins of people blood increased, that most probably connected with disturbing of ferroprotein metabolism. Maximum intensity values had people, irradiated with low doses (to 2 cGy), that corroborate the theory of danger these doses for the people organism.